FIG. 2.

Analysis of G₀ transgenic chickens expressing scFv-Fc. (a) Structure of the MSCV/GΔAscFv-Fc retroviral vector. GFP, green fluorescent protein gene; scFv-Fc, chicken antiprion single-chain antibody fragment gene joined with the Fc region gene from human immunoglobulin G1. The other abbreviations are the same as those for the MSCV/NΔAβ vector (Fig. 1a). (b) PCR analysis for detection of the transgene. The retroviral vector sequences were amplified by PCR using the primers for GFP (upper) or scFv-Fc (lower) and genomic DNA obtained from the tissues (brain, heart, liver, muscle, and testis) and sperm of a transgenic rooster as a template. WT, wild type; TG, transgenic; PC, positive control (pMSCV/GΔAscFv-Fc plasmid as a template). (c) Western blot analysis for transgene expression (scFv-Fc and GFP) in tissues from an adult male (brain, heart, liver, muscle, and testis). scFv-Fc or GFP produced by recombinant CHO cells was used as a positive control (PC). (d) Long-term production of scFv-Fc protein in eggs laid by G₀ transgenic hens.