FIG. 4.

Western blot and SDS-PAGE analyses of scFv-Fc produced by transgenic chickens. (a to c) Western blot analysis for scFv-Fc expression in serum (a), egg white (b), and yolk (c) produced by G₀ and G₁ transgenic hens. WT, wild type; PC, positive control (30 ng of purified scFv-Fc produced by recombinant CHO cells). (d and e) SDS-PAGE analysis of scFv-Fc under reducing and nonreducing conditions in the serum (d) and egg white (e) of a G₀ transgenic hen. (f) Antigen recognition of scFv-Fc by Western blot analysis. The natural prion proteins in mouse brain homogenate (mB) were detected by Western blotting using the scFv-Fc purified from the egg white produced by a transgenic chicken. NC, negative control (GST); PC, positive control (GST-PrP) (28).