TABLE 2.
List of representative G0 chickens
| Bird no. | Viral titer (IU/ml) | Injection volume (μl) | Sex (M, male; F, female) | scFv-Fc concna(mg/ml)
|
||
|---|---|---|---|---|---|---|
| Serum | White | Yolk | ||||
| 102 | 6.0 × 108 | 2.5 | F | 0.24 ± 0.13 | 0.06 ± 0.02 | 0.04 ± 0.02 |
| 105 | 2.0 | M | 0.29 ± 0.18 | |||
| 108 | 3.0 | F | 0.40 ± 0.18 | 0.59 ± 0.22 | 0.10 ± 0.04 | |
| 110 | 2.5 | F | 1.72 ± 0.34 | 3.88 ± 1.32 | 0.85 ± 0.23 | |
| 111 | 2.0 | M | 1.74 ± 0.46 | |||
| 116 | 2.5 | M | 0.82 ± 0.35 | |||
| 305 | 1.2 × 109 | 2.5 | F | 2.08 ± 0.44 | 4.85 ± 1.25 | 1.18 ± 0.32 |
| 308 | 3.5 | M | 1.73 ± 0.45 | |||
| 402 | 1.8 × 109 | 1.5 | M | 1.60 ± 0.37 | ||
| 405 | 2.5 | M | 3.50 ± 0.70 | |||
| 407 | 1.5 | F | 1.97 ± 0.64 | 4.00 ± 0.84 | 0.81 ± 0.25 | |
| 410 | 3.0 | F | 3.56 ± 0.93 | 5.57 ± 1.36 | 1.77 ± 0.38 | |
a
The scFv-Fc concentration was determined by solid-phase enzyme-linked immunosorbent assay using purified scFv-Fc or human Fc fragment as the standard.