TABLE 3.
Generation of transgenic progeny expressing antiprion scFv-Fc
| Transgenic chickens | Parental chicken | Bird no. | Sex (M, male; F, female) | scFv-Fc concn (mg/ml)
|
Transgene copy numbera | Chromosomal locationa | Germline transmission efficiency | ||
|---|---|---|---|---|---|---|---|---|---|
| Serum | White | Yolk | |||||||
| G1 | G0 no. 402 | 1 | M | <1 × 10−6 | 1 | Micro | NDb | ||
| 2 | F | 1.12 ± 0.16 | 0.22 ± 0.06 | 0.48 ± 0.15 | 2 | Micro × 2 | 21/27 (78%) | ||
| 3 | M | 0.51 ± 0.10 | 1 | 3q2.6-2.8 | 22/43 (51%) | ||||
| 4 | F | 0.50 ± 0.15 | 0.41 ± 0.13 | 0.22 ± 0.12 | 1 | 3q2.6-2.8 | ND | ||
| 5 | F | 1.94 ± 0.57 | 1.50 ± 0.24 | 0.76 ± 0.20 | 1 | Micro | ND | ||
| 6 | F | 0.47 ± 0.03 | 0.43 ± 0.12 | 0.17 ± 0.05 | 1 | 3q2.6-2.8 | ND | ||
| G2 | G1 no. 2 | 1 | F | 0.68 ± 0.15 | 0.10 ± 0.05 | 0.24 ± 0.14 | 2 | ||
| 2 | F | 0.56 ± 0.09 | 0.05 ± 0.01 | 0.52 ± 0.07 | 2 | ||||
| 3 | F | 0.76 ± 0.14 | ND | ND | 2 | ||||
| 4 | F | 0.30 ± 0.10 | ND | ND | 1 | ||||
| 5 | M | 0.20 ± 0.07 | 1 | ||||||
| 6 | M | 0.29 ± 0.03 | 1 | ||||||
| 7 | M | 0.20 ± 0.08 | 1 | ||||||
| 8 | M | 0.25 ± 0.09 | 1 | ||||||
| G2 | G1 no. 3 | 9 | F | 0.30 ± 0.10 | 0.16 ± 0.05 | 0.18 ± 0.06 | 1 | ||
| 10 | F | 0.41 ± 0.13 | 0.36 ± 0.09 | 0.26 ± 0.10 | 1 | ||||
| 11 | F | 0.30 ± 0.10 | 0.17 ± 0.05 | 0.21 ± 0.06 | 1 | ||||
| 12 | M | 0.38 ± 0.09 | 1 | ||||||
| 13 | M | 0.38 ± 0.09 | 1 | ||||||
| 14 | M | 0.33 ± 0.13 | 1 | ||||||
a
Transgene copy number and chromosomal location of the transgene were determined by FISH and/or Southern blot analyses.
b
ND, not determined.