FIG. 10.
PRV Bartha but not gE, gI, or Us9 null single mutants have a slight defect in axon-mediated infection of neurons. A: High-titer viral inoculum from PRV Becker and PRV Bartha were incubated in the N-compartment for 1 hour before being replaced with regular medium. At 24 h postinfection, infected neuron cell bodies in the S-compartment were harvested, lysed, and titered on PK15 cells. A total of five chambers were used for each type of infection. The standard deviations are: S-compartment (Becker, ±1.2 × 105, Bartha, ±2.1 × 103). B: Immunofluorescence experiment on axon mediated infection of neurons by PRV Becker. Confocal microscopy images show the S- (a to c), M- (d to f), and N-compartments (g to i) being labeled with either antibodies against VP5 (a, d, and g), or DiI (b, e, and h). Merged images are shown in panel B (c, f, and i). Scale bar: 20 μm. C: High-titer viral inocula from PRV Becker, PRV 758, PRV 98, and PRV 160 were incubated in the N-compartment for 1 hour before being replaced with regular medium. At 24 h postinfection, infected neuron cell bodies in the S-compartment were harvested, lysed, and titered on PK15 cells. A total of five chambers were used for each type of infection. The standard deviations are: S-compartment (Becker, ±5.9 × 105; 758, ±9.1 × 105; 160, ±5.7 × 105; 98, ±6.7 × 105). The open circle beside each data set shown in the scatter plots represents the average value for that particular set of data.