Neuron-to-cell spread of infection is not cell type specific. PK15 cells, Madin-Darby bovine kidney cells, pig embryonic fibroblasts, and rat embryonic fibroblasts were plated in the N-compartment. Neurons in the S-compartment were then infected with PRV Becker at high MOI for 48 h. Infected cells were then harvested from both the S- and N-compartments and titered on PK15 cells. Six chambers were used for each type of infection. The standard deviations are: N-compartment (PK15 cells, ±1.6 × 108, MDBK cells, ±6.6 × 105, pig embryonic fibroblasts, ±2.8 × 106, rat embryonic fibroblasts, ±2.4 × 106); S-compartment (PK15 cells, ±8.3 × 105, MDBK cells, ±8.2 × 105, pig embryonic fibroblasts, ±2.7 × 105, rat embryonic fibroblasts, ±7.8 × 105). The open circle beside each data set represents the average value for that particular set of data.