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. 2005 Sep;79(17):11259–11268. doi: 10.1128/JVI.79.17.11259-11268.2005

FIG. 4.

FIG. 4.

Effect of Ads on NF-κB and AP-1 transcription factor activity. (A) Degradation of IκBα (inset) and the induction of TNF-α production in response to LPS stimulation of PBMCs in the presence of different Ad vectors were analyzed as described in Materials and Methods. (B and C) Cells were harvested 30 min after LPS addition for lκBα analysis by Western blotting, while replicate culture supernatants were collected and assayed for TNF-α. AP-1-driven luciferase reporter gene activity, expressed as relative light units (RLU), was measured in transfected HeLa (B) and THP-1 (C) cells following stimulation with PMA and ionomycin.