FIG. 4.

The axonal transport of the wt proteins was assayed by Western blotting of segments of the optic pathway. The envelope protein, gD (left panel), was detected in the ON1 and ON2 segments of the optic pathway at 3 dpi. The capsid protein, VP5 (right panel), was detected throughout the pathway by 3 dpi. gD was detected in ON, ON2, and the OC by 4 dpi. VP5 persisted throughout the pathway. (+), viral stock aliquots; (−), optic pathway homogenates from uninfected animal. Markers = 50 kDa (left panels) and 155 kDa (right panels).