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. 2005 Sep;79(17):10902–10914. doi: 10.1128/JVI.79.17.10902-10914.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 4. — Immunization schedule. Schematic representation of the immunization regimens used in the mouse and rabbit experiments. (A) Mice (six per group) were immunized three times with 10 μg purified YU2gp140(-/GCN4) protein in Ribi (P) or two or three times with 10⁷ IU rSFV-YU2gp140(-/GCN4) particles (SFV), followed by 10 μg purified YU2gp140(-/GCN4) protein in Ribi at the indicated intervals. Four control mice were immunized two times with 10⁷ rSFV-lacZ (SFVlacZ) followed by 10 μg βgal in Ribi. (B) Rabbits (four per group) were immunized three times with 25 μg purified YU2gp140(-/GCN4) protein in Ribi (P) or two times with 5 × 10⁷ IU rSFV-YU2gp140(-/GCN4) particles (SFV), followed by 25 μg or 5 μg purified YU2gp140(-/GCN4) protein in Ribi at the indicated intervals. Two control rabbits were immunized two times with 5 × 10⁷ rSFV-lacZ (SFVlacZ) followed by 25 μg BSA in Ribi.