TABLE 1.
Indirect immunofluorescence assay of the reactivity of monoclonal antibodies with acetone-permeabilized cells transfected with plasmids expressing gL and either wild-type gH or one of the 13 insertion mutants (M1 to M13)a
| gH construct | Reactivity to:
|
|||
|---|---|---|---|---|
| Anti-Flag | E1D1 | CL40 | CL59 | |
| Wild type | ++ | ++ | ++ | ++ |
| M1 | ++ | ++ | ++ | ++ |
| M2 | ++ | ++ | ++ | ++ |
| M3 | ++ | ++ | ++ | ++ |
| M4 | ++ | ++ | ++ | ++ |
| M5 | ++ | ++ | ++ | ++ |
| M6 | ++ | ++ | ++ | ++ |
| M9 | ++ | ++ | ++ | ++ |
| M10 | ++ | ++ | ++ | ++ |
| M11 | ++ | ++ | ++ | ++ |
| M7 | + | +/− | +/− | +/− |
| M13 | + | + | + | + |
| M8 | + | − | − | − |
| M12 | + | − | − | − |
a
Cell staining was scored as very bright (++), bright (+), weak (+/−), or absent (−).