FIG. 6.

Partitioning of MHV N, M, and ORF6-HA proteins into hydrophobic and hydrophilic environments. 17Cl-1 cells infected with rJ.2.2.6 were collected at 16 h p.i., and proteins were partitioned into hydrophobic (Triton X-114 detergent) and hydrophilic (aqueous) phases, using M-PER reagents (Pierce Company, Rockford, IL). Proteins from the indicated cell equivalents were then subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to polyvinylidene difluoride membranes, and probed simultaneously with anti-N MAb 5A5.2 and Covance anti-HA MAb 1.1 (top panel). The blot was then stripped and reprobed with anti-M MAb 4B6.2 (bottom panel).