TABLE 3.
Summary of Ad.HCA-CMV-GFP integration site analysis in MO7e clonesa
| Clone | 5′ or 3′ | Ad ITR (bp) | XX (bp) | Integration (chromosome) | Integration (genome position) | Integrate into gene | Gene discription |
|---|---|---|---|---|---|---|---|
| 52/1 | 5′ | 6 | 17 | 27,353,505 | |||
| 15/1 | 3′ | 1 | 15 | 68,288,799 | − | − | |
| 18/2 | 3′ | 3 | X | 149,299,948 | CXorf6 | Chromosome X open reading frame 6; intron | |
| 22/5 | 3′ | 2 | 2 | 7,617,172 | − | − | |
| 40/5 | 3′ | 4 | 19 | 44,439,468 | − | − | |
| 41/1 | 3′ | 17 | 5 | 1 | 199,630,961 | ||
| 52/3 | 3′ | 1 | 6 | 3,262,660 | C6orf85 | Ion transporter protein; intron | |
| 65/3 | 3′ | 2 | 19 | 4,068,455 | MAP2k2 | Mitogen-activated protein kinase kinase 2; exon | |
| 69/1 | 3′ | 10 | 1 | 17 | 57,022,779 | ANAC | Alpha-NAC protein; exon |
| 69/7 | 3′ | 5 | 7 | 82,304,970 | − | − | |
| 73/2 | 3′ | 3 | 9 | 131,133,585 | NUP214 | Nucleoporin 214-kDa; intron | |
| 97/3 | 3′ | 10 | 6 | 140,127,347 | − | − | |
| 98/1 | 3′ | 2 | 1 | 54,402,564 | − | − | |
| 98/2 | 3′ | 20 | 4 | 149,457,240 | NR3C2 | Nuclear receptor subfamily 3 group C member 2; intron | |
| 98/3 | 3′ | 76 | 145 | 14 | 99,794,430 | YY1 | YY1 transcription factor; intron |
| 22/2 | 5′ | 630 | 10 | 67,881,795 | GRID1 | Glutamate receptor, ionotropic delta 1; intron | |
| 22/5 | 5′ | 630 | 17 | 49,687,702 | − | − | |
| 24/4 | 5′ | 630 | 10 | 125,243,823 | |||
| 52/1 | 5′ | 783 | 19 | 44,598,568 | PLEKHG2 | Pleckstrin homology domain containing, family G; intron | |
| 75-1/4 | 5′ | 630 | 21 | 33,339,549 | − | − | |
| 75-2/1 | 5′ | 630 | 146 | 1 | 205,929,122 | − | − |
| 75-2/2 | 5′ | 630 | 9 | 133,703,436 | VAV2 | Vav 2 oncogene; intron | |
| 75-2/3 | 5′ | 630 | 17 | 29,213,176 | ACCN1 | Neuronal amiloride-sensitive cation channel 1; intron |
a
Analysis of integration sites was conducted as described for Ad.LCR-β-GFP.