FIG. 5.

Vpr partially rescues Δvif HIV-1 infectivity. (A) Wild-type, Δvif, Δvpr, and Δvif Δvpr luciferase reporter viruses were produced in 293T cells cotransfected with the indicated amounts of human APOBEC3G expression vector. Luciferase activities generated by infection of HOS cells with 1 ng of each virus are shown. The error bars indicate standard deviations. (B) Same as panel A, but infectivity was measured in PBMCs. (C) Reporter viruses were generated in cells cotransfected with wild-type or W54R Vpr in trans with or without human APOBEC3G, and their infectivities were determined by luciferase assay. The data shown are representative of three independent repetitions of the experiment. (D) HOS.CD4.X4 (left) or HOS.CD4.X4 APOBEC3G.HA (right) cells were infected with the indicated viruses at a multiplicity of infection of 0.1. Virus production was determined at the indicated time points by p24 ELISA.