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. 2004 Jan 5;199(1):25–34. doi: 10.1084/jem.20020509

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Copyright © 2004, The Rockefeller University Press

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Figure 2. — Flow cytometric analysis of wild-type, asymptomatic, or paralyzed STAT1-deficient mice. (A) Cells derived from lymph nodes of wild-type, asymptomatic, or paralyzed STAT1-deficient animals were stained for CD4, CD69, and Vβ8. Horizontal axis represents Vβ8 staining, vertical axis corresponds to CD4 (top) or CD69 (bottom) staining of CD8⁻ cells. Cells displayed in lower panels are CD4⁺. (B) Splenocytes derived from wild-type, asymptomatic, or paralyzed STAT1-deficient animals were stimulated with 50 ng/ml PMA/500 ng/ml ionomycin for 4 h (10 μg/ml brefeldin A was added during the last 2 h) and stained for CD4, CD8, and intracellular IFN-γ. CD4 staining (horizontal axis) and IFN-γ production (vertical axis) of CD8⁻ cells is shown.