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. Author manuscript; available in PMC: 2006 Jul 8.
Published in final edited form as: J Biol Chem. 2005 May 9;280(27):25849–25853. doi: 10.1074/jbc.M413721200

FIGURE 3. Time course, dose-dependence and effect of protein kinase inhibitors on IFN induced NF-κB DNA binding activity in JAK1− fibrosarcoma cells.

FIGURE 3

U4A cells were either (Panel A) treated with IFN (1000 IU/ml) for the indicated times; (Panel B) treated with the indicated concentrations of IFN for 30 min; or (Panel C) pretreated with either DMSO as vehicle (Veh), the tyrosine kinase inhibitor genistein (100 μM), the PI-3K inhibitor LY294002 (10 μM), or the general protein kinase inhibitor staurosporin (100 nM) for 1 hr prior to IFN addition (1000 IU/ml, 30 min). Nuclear extracts were subjected to EMSA with a [32P]-labeled κB oligonucleotide probe.