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. Author manuscript; available in PMC: 2006 Jul 8.
Published in final edited form as: J Biol Chem. 2005 May 9;280(27):25849–25853. doi: 10.1074/jbc.M413721200

FIGURE 6. The role of TYK2 in JAK1-independent NF-κB activation by IFN.

FIGURE 6

JAK1− U4A cells (Panels A and B) and Daudi cells (Panels C and D) were transiently transfected by electroporation with either empty vector (EV), TYK2 (WT) or the K930R TYK2 mutant (KD). After 48 hr the cells were treated with IFN (1000 IU/ml) for 30 min and either total cell lysates were prepared analyzed by immunoblotting for phospho-TYK2, TYK2 and actin (Panels A and C), or nuclear extracts were prepared and subjected to EMSA with a [32P]-labeled κB oligonucleotide probe (Panels B and D).