FIGURE 2.

Protein expression of AFI axis family members in the bladder of male and female mice. (A–G) Western blot analysis of INHA (A), INHBA (B), INHBE (C), FST (D), ACVR1 (E), ACVR1B (F), ACVR2B (G) in both male and female mouse bladder (25 μg protein loaded). (H) Differences in protein expression of AFI axis family members in the bladder of male and female mice. (n = 4, molecular values of major detected protein species are indicated at the right of each panel. Data were analyzed with the use of a two-tailed Student’s t-test, **p < 0.01, ***p < 0.001).