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. 2005 Sep 1;115(9):2363–2372. doi: 10.1172/JCI23874

Figure 3.

Figure 3

Lymphatic vascular endothelium after PKP and suture placement. (A–F) Fluorescence microscopy. FITC-conjugated CD11b (A) and Cy3-visualized LYVE-1 (B) staining. (C) Overlay of A and B showing dual expression of markers. FITC-conjugated CD11c (D) and Cy3-visualized LYVE-1 (E) staining. (F) Overlay of D and E showing dual expression. (G–T) Confocal microscopy. FITC-conjugated CD11b (G) and Cy3-visualized Prox-1 (H) staining. (I) Overlay of G and H showing dual expression. FITC-conjugated CD11b (J) and Cy3-visualized Prox-1 (K) staining. (L) Overlay of G and K showing dual expression. (M) Horizontal section image (bottom) and coronary section image (right) of the lymphatic vessel. (N–Q) EGFP bone marrow cells (green) appear to be part of the lymphatic endothelium or wall. EGFP expression (N) and Cy3-visualized LYVE-1 (O) staining. (P) Overlay of N and O showing dual expression. (Q) Horizontal section image (bottom) and coronary section image (right) of the lymphatic wall. (R–T) Three-dimensional image of a lymphatic vessel. EGFP expression (R) and Cy3-visualized LYVE-1 (S) staining. (T) Overlay of P and R showing dual expression. Arrows indicate lymphatic vessel lumen. Magnification, ×100 (A–F). Scale bars: 16 μm (G–M); 8 μm (N–Q).