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. 1987 Feb;63(1):22–25. doi: 10.1136/sti.63.1.22

Evaluation of single whole inclusion serum test for IgG antibody to Chlamydia trachomatis in asymptomatic women.

M P Meyer, A J Amortegui
PMCID: PMC1194001  PMID: 3817822

Abstract

IgG antibody titres against Chlamydia trachomatis were measured using a whole inclusion assay (Electro-Nucleonics Laboratories, Bethesda, Maryland, USA) in single serum samples of patients who presented for termination of pregnancy with evidence of infection with this organism (case group) and of 30 women with no evidence of infection (control group). Case and control patients were matched for age, race, marital status, history of sexually transmitted diseases, number of lifetime sexual partners, and whether they had had a new sexual partner in the preceding month. Fifty eight of 60 (97%) serum samples tested had chlamydial antibodies present at a titre of 1/8 or more. There was no significant difference between the geometric mean titres of the 30 case patients and the 28 controls with chlamydial antibodies (1/161.3 and 1/77.4 respectively). Significantly more case patients than controls, however, had a titre of 1/128 or more (p less than 0.01). The sensitivity, specificity, and positive and negative predictive values of a single serum antibody titre of 1/128 or more as an indicator of recent infection compared with isolation of the organism in cycloheximide treated McCoy cells or antigen detection by Chlamydiazyme (Abbot Laboratories; North Chicago, Illinois, USA), or both, were each 67%. The value of the antibody assay seems to be questionable as high levels of antibodies failed to correlate with isolation or antigen detection results, or both, in our population.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amortegui A. J., Meyer M. P. Enzyme immunoassay for detection of Chlamydia trachomatis from the cervix. Obstet Gynecol. 1985 Apr;65(4):523–526. [PubMed] [Google Scholar]
  2. Beem M. O., Saxon E. M. Respiratory-tract colonization and a distinctive pneumonia syndrome in infants infected with Chlamydia trachomatis. N Engl J Med. 1977 Feb 10;296(6):306–310. doi: 10.1056/NEJM197702102960604. [DOI] [PubMed] [Google Scholar]
  3. Inouye S., Kono R., Takeuchi Y. Oligomeric immunoglobulin A antibody response to rubella virus infection. J Clin Microbiol. 1978 Jul;8(1):1–6. doi: 10.1128/jcm.8.1.1-6.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kordová N., Wilt J. C., Sekla L., Wenman W. M., Stackiw W., Feltham S. New commercial chlamydial antigens in the serology of chlamydial infections in a selected population of the province of Manitoba. Can J Public Health. 1982 Nov-Dec;73(6):424–426. [PubMed] [Google Scholar]
  5. Munday P. E., Johnson A. P., Thomas B. J., Taylor-Robinson D. A comparison of the sensitivity of immunofluorescence and Giemsa for staining Chlamydia trachomatis inclusions in cycloheximide-treated McCoy cells. J Clin Pathol. 1980 Feb;33(2):177–179. doi: 10.1136/jcp.33.2.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mårdh P. A., Helin I., Bobeck S., Laurin J., Nilsson T. Colonisation of pregnant and puerperal women and neonates with Chlamydia trachomatis. Br J Vener Dis. 1980 Apr;56(2):96–100. doi: 10.1136/sti.56.2.96. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Møller B. R., Ahrons S., Laurin J., Mårdh P. A. Pelvic infection after elective abortion associated with Chlamydia trachomatis. Obstet Gynecol. 1982 Feb;59(2):210–213. [PubMed] [Google Scholar]
  8. Osser S., Persson K. Postabortal pelvic infection associated with Chlamydia trachomatis and the influence of humoral immunity. Am J Obstet Gynecol. 1984 Nov 15;150(6):699–703. doi: 10.1016/0002-9378(84)90670-7. [DOI] [PubMed] [Google Scholar]
  9. Qvigstad E., Skaug K., Jerve F., Fylling P., Ulstrup J. C. Pelvic inflammatory disease associated with Chlamydia trachomatis infection after therapeutic abortion. A prospective study. Br J Vener Dis. 1983 Jun;59(3):189–192. doi: 10.1136/sti.59.3.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Qvigstad E., Skaug K., Jerve F., Vik I. S., Ulstrup J. C. Therapeutic abortion and Chlamydia trachomatis infection. Br J Vener Dis. 1982 Jun;58(3):182–183. doi: 10.1136/sti.58.3.182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Richmond S. J., Caul E. O. Fluorescent antibody studies in chlamydial infections. J Clin Microbiol. 1975 Apr;1(4):345–352. doi: 10.1128/jcm.1.4.345-352.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schachter J., Cles L., Ray R., Hines P. A. Failure of serology in diagnosing chlamydial infections of the female genital tract. J Clin Microbiol. 1979 Nov;10(5):647–649. doi: 10.1128/jcm.10.5.647-649.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Stamm W. E., Tam M., Koester M., Cles L. Detection of Chlamydia trachomatis inclusions in Mccoy cell cultures with fluorescein-conjugated monoclonal antibodies. J Clin Microbiol. 1983 Apr;17(4):666–668. doi: 10.1128/jcm.17.4.666-668.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tam M. R., Stamm W. E., Handsfield H. H., Stephens R., Kuo C. C., Holmes K. K., Ditzenberger K., Krieger M., Nowinski R. C. Culture-independent diagnosis of Chlamydia trachomatis using monoclonal antibodies. N Engl J Med. 1984 May 3;310(18):1146–1150. doi: 10.1056/NEJM198405033101803. [DOI] [PubMed] [Google Scholar]

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