Figure 9.

Viability of human hepatocellular carcinoma cells (HepG2 cell line) after 24 h incubation with mixtures of bicyclic monoterpenes, which exhibited the best activity during metal ion reduction and chelation assays. The compounds were used in non-cytotoxic concentrations, i.e., 0.312 mM. Metabolic activity was evaluated using the MTT assay, and results were presented as a percentage value compared to the control. Confocal microscope images were taken after staining cells with the Live/Dead Double Staining Kit–live cells emitted green fluorescence, while dead cells generated red; Magnification ×100, scale bar = 150 μm.