Table 2.

Muscle-free amino acid levels in Litopenaeus vannamei (mg/100g).

Amino acids	Treatments
	PRLA0	PRLA30	PRLA60	PRLA90
Thr	17.66 ± 1.75	20.39 ± 3.16	20.60 ± 2.49	20.19 ± 0.46
Val	33.04 ± 1.48	26.50 ± 2.66	28.46 ± 2.19	32.70 ± 1.12
Leu	26.12 ± 2.48	26.15 ± 3.18	27.75 ± 2.67	30.28 ± 0.14
Ile	17.90 ± 1.41	16.97 ± 1.73	16.95 ± 1.64	18.13 ± 0.38
Met	3.66 ± 0.03	3.72 ± 0.15	3.37 ± 0.54	3.54 ± 0.30
Phe	12.64 ± 0.96	13.60 ± 0.88	11.95 ± 1.40	13.17 ± 0.02
Lys	20.21 ± 0.55	25.11 ± 4.12	23.21 ± 2.70	24.93 ± 0.50
ΣEAA	131.22 ± 7.28	132.45 ± 15.72	132.29 ± 12.97	142.95 ± 1.07
Asp	4.55 ± 0.37ab	5.03 ± 0.29b	3.75 ± 0.22a	5.10 ± 0.28b
Glu	37.78 ± 0.54ab	38.13 ± 1.59b	33.88 ± 1.59a	39.75 ± 0.91b
Gly	150.93 ± 1.04a	164.93 ± 5.00b	164.29 ± 2.72b	166.45 ± 4.70b
Ala	169.97 ± 2.77	180.67 ± 18.02	165.63 ± 6.90	177.00 ± 8.21
ΣFAA	363.23 ± 1.46a	388.76 ± 2.67b	367.54 ± 5.86a	388.29 ± 5.22b
Ser	2.25 ± 0.10	2.23 ± 0.11	2.10 ± 0.13	2.25 ± 0.08
Tyr	16.47 ± 0.28	17.68 ± 1.44	18.71 ± 0.10	19.11 ± 0.45
His	25.11 ± 2.71	24.82 ± 0.96	22.16 ± 1.60	25.66 ± 1.07
Arg	471.09 ± 3.78a	464.49 ± 5.24a	464.26 ± 17.78a	510.99 ± 1.43b
Pro	285.50 ± 17.25a	309.70 ± 22.92ab	337.00 ± 5.83ab	355.92 ± 19.69b
ΣAA	1294.87 ± 27.79a	1340.14 ± 10.96a	1344.06 ± 26.01a	1445.16 ± 24.75b

Note: Data are represented by means ± standard error (n = 3). Means in one column with diverse superscripts show significant differences (p < 0.05).

Abbreviations: ΣAA, total amino acids; ΣEAA, total essential amino acids; ΣFAA, total flavor amino acids.