Fig. 4.

siRNA mediated down-regulation of FADD results in decrease in NF-κB activity. (A) SKLU-1 lysates from cells transfected with FADD siRNA and scramble siRNA and nontransfected control cells were subjected to Western blot analyses using antibodies to FADD, I-κB, and cyclins D1 and B1. Antibody to actin was used as a control. (B) Nuclear lysates were made from SKLU-1 cells transfected with FADD siRNA and scramble siRNA and from nontransfected control cells and were subjected to NF-κB activity by using the TransAM NF-κB kit from Active Motif. Data shown is derived from mean (±SD) of relative NF-κB activity to nontransfected controls of three separate experiments, performed in triplicate