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Both PTN and PMA enhance phosphorylation of β-adducin residue 713 and α-adducin residue 724. (Upper) Western blot of lysates from PTN-treated (50 ng/ml, for 15 and 60 min), PMA-tretaed (1 μM, 60 min), or PKCα/β inhibitor-treated (60 min before PTN and PMA treatment) HeLa cells probed with anti-phosphoserine 713 β-adducin and anti-phosphoserine 724 α-adducin antibodies. (Lower) Reprobe of Western blot with an anti-actin-specific antibody.
