Fig. 3.

Gq mediates the inhibitory effect of ET-1 on neural progenitor migration. (A) Neural progenitor cells were infected with adenoviruses harboring GFP, Gαq-ct, and Gαi-ct at a multiplicity of infection of 25. Forty-eight hours after infection, neurospheres were plated on cover glasses coated with PDL and laminin and cultured for 24 h. Representative phase-contrast images are shown. (B) The effect of Gαq-ct and Gαi-ct on the migration of neural progenitor cells is quantitated as a percentage of migrating neurospheres. Data are shown as the mean ± SD of at least three independent experiments. (Bar, 250 μm.)