TABLE 2.
Secondary antibodies used for IF staining. The presented dilutions were half-reduced for immunohistochemical stainings.
| Antigen | Fluorochrome | Isotype | Dilution | Manufacturer | Catalog number |
|---|---|---|---|---|---|
| Alexa Fluor Goat (anti-rabbit) | Alexa 488 | IgG | 1:1,000 | Life Technologies | A11034 |
| Alexa Fluor Goat (anti-rabbit) | Alexa 546 | IgG | 1:1,000 | Life Technologies | A11035 |
| Alexa Fluor Goat (anti-mouse) | Alexa 546 | IgM | 1:1,000 | Life Technologies | A21123 |
| Alexa Fluor Goat (anti-mouse) | Alexa 546 | IgG2b | 1:1,000 | Life Technologies | A21143 |
| ALEXA FLUOR GOAT (ANTI-RABBIT) | Alexa 647 | IgG | 1:1,000 | Life Technologies | A21245 |
| ALEXA FLUOR (ANTI-GOAT) | Alexa 546 | IgG | 1:1,000 | Life Technologies | A11056 |