Fig. 1. Overview of the μSonic-hand.

(A) μSonic-hand includes an additional piezoelectric transducer in a typical cell injection unit under the optical inverted microscope. (B) The gas-liquid interface oscillates in response to the acoustic waves emitted by the piezoelectric transducer, generating 3D axisymmetric streaming. (C) The generated local acoustic streaming with a 3D axisymmetric vortex pattern is visualized by the 1-μm polystyrene (PS) fluorescent particles (movie S1). (D) The acoustic radiation force enables the trapping and 3D transportation of cross-scale objects at the microscale. (E) Multidirectional rotation by altering the object’s relative position in the axisymmetric vortex. rps, revolutions per second.