Table 1. Description of the labeling pattern in each class.

Class	Replication timing*	Image†	Labeling pattern
IA	~2 hours after the S-phase onset		Throughout the nucleoplasm, excluded from the nuclear and nucleolar periphery
IB	~4 hours after the S-phase onset		Throughout the nucleus, including the nuclear and nucleolar periphery
II	~6 hours after the S-phase onset		Nuclear and nucleolar periphery
III‡	~7 hours after the S-phase onset		Large and discrete dot-like foci at the nucleoplasm and periphery
Ambiguous (Fig. 9 and fig. S13)	Appears in RIF1-depleted cells with aberrant replication timing		Not categorized into any of the four classes, and distributed uniformly throughout the nucleus

*Replication timing was determined by cell cycle–synchronized HeLa cells.

†Scale bar, 5 μm.

‡Class III H3.2-Halo also shows diffusive signals that are not colocalized with nascent DNA with EdU incorporation (fig. S5B).