Figure 3.

Super LKB1 enhances the oxidative metabolism in A549-edited cells.A, Western blotting of HK2, LDHA, and GAPDH in cWT, c2SL+, and c3SL + cells; (B) normalized levels of proteins by GAPDH (a.u); (C) Western blotting of OXPHOS and GAPDH in cWT, c2SL+, and c3SL + cells. D, normalized levels of proteins by GAPDH (a.u); (E) oxygen consumption rate (OCR) by OROBOROS in cWT, c2SL+, and c3SL + cells; (F) comparison levels of O₂ in the cell lines in the basal condition and after treatments with oligomycin and FCCP. The data are presented as mean ± SD. Statistical analysis was performed by ANOVA followed by Dunnet’s test ∗p < 0.05, ∗∗p < 0.01. These data are representative of two independent experiments.