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. 2025 Feb 13;301(3):108308. doi: 10.1016/j.jbc.2025.108308

Figure 4.

Figure 4

The Super LKB1 inhibits mTORC1 effectors kinases, S6K2 and S6, impacting translation and proliferation activity.A, Western blotting of pmTOR (S2448), mTOR, pS6K1 (T389), S6K1, pS6K2 (S423), S6K2, pS6 (S240/244), S6, and GAPDH in cWT, c2SL+, and c3SL + cells; (B) normalized levels of proteins by GAPDH (a.u); (C) surface sensing of translation (SUnSET) by Puromycin treatment followed by Western blotting to detect puromycin-labeled amino acids in cWT, c2SL+, and c3SL + cells; (D) normalized levels of protein by GAPDH (a.u); (E) proliferation curve beginning with 1.104 cells and counting for 72 h in cWT, c2SL+, and c3SL + cells. Data are presented as mean ± SD. Statistical analysis has been performed by ANOVA followed by Dunnet’s test ∗p < 0.05, ∗∗p < 0.01. These data are representative of two independent experiments.