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. 2025 Feb 13;301(3):108308. doi: 10.1016/j.jbc.2025.108308

Figure 5.

Figure 5

The A549-edited cells show an increase in autophagy flux due to Super LKB1 expression.A, Western blotting of pULK (S555), ULK, p62, LC3 I/II, and GAPDH in cWT, c2SL+, and c3SL + cells. B, normalized levels of proteins by GAPDH (a.u). C, autophagy flux detection with ptfLC3 plasmid being LC3 GFP autophagy substrate and LC3 RFP internal control in cWT, c2SL+, and c3SL + cells, the highlighted square evidence the fluorescent dots; (D) normalized RFP/GFP in cWT, c2SL+, and c3SL + cells. The higher ratio indicates an increase in autophagy flux. The data are presented as mean ± SD. Statistical analysis was performed by ANOVA followed by Dunnet’s test ∗p < 0.05. These data are representative of two independent experiments.