Fig. 2.

6-OHDA induced unilateral lesion model of Parkinson’s disease shows loss of TH expression (DA biosynthesis) in SN and STR but not in PFC. (A) Number of contralateral rotations induced by apomorphine (0.5 mg/kg; s.c) in sham (n = 8) and PD (n = 12) rats. Unpaired t-test showed a significant increase (t18 = 4.443, ***P < 0.001) in rotation in PD group of animals compared to Sham. Representative western blot images and relative expression of tyrosine hydroxylase (TH) protein quantified in sham and lesioned rats treated with saline in (B) SN (t4 = 3.794, *p < 0.05, n = 3 rats/gp); (C) STR (t4 = 3.169, *p < 0.05,) and (D) PFC (not significant). Western blots were quantified as relative levels to the control protein β-actin. Data are represented as Mean ± S.E.M and unpaired t-test was performed to test the significance