FIG. 1.

gag processing monitored by p24 Western blotting. The accumulation of p55 (open), p41 (hatched), p25 (stippled), and p24 (wavy) is plotted as a percentage of total p24-related protein. Cells were transfected with NL4-3 gag-pol expression vectors, and replicate samples were harvested after 6, 12, and 24 h. Western blots of total cell lysates were probed with anti-p24 MAb, followed by quantitation of individual protein bands, as described in Materials and Methods. (A) Wild-type (WT) gag-pol; (B) I50L gag-pol; (C) A71V gag-pol; (D) I50L+A71V gag-pol.