TABLE 3.
Summary of antibacterial and hemolytic activities of Flo and derivativesa
| Peptide | Conc (μM) | Surviving cells (%) | Hemolytic activity (%) |
|---|---|---|---|
| Flo | 300 | 10−2-10−1 | 10 |
| 30 | 100 | ND | |
| P4a | 300 | 10−4-10−3 | 41 |
| P4d | 300 | 10−5-10−3 | 34 |
| P4a MAP | 300 | <10−11 | 22 |
| 30 | 10−4-10−3 | 0 | |
| 3 | 10−1 | 0 | |
| 0.3 | 10-100 | 1 | |
| P4d MAP | 300 | <10−11 | 19 |
| 30 | 10−4-10−3 | 6 | |
| 3 | 10−1-1 | 0 | |
| 0.3 | 100 | 1 |
a
The antibacterial effect was determined on E. coli and depicts the percentage of surviving cells after treatment. Nontreated cultures (100%) correspond to an average of 1012 bacteria per assay. Hemolytic activity was determined by recording the absorbance of red blood cell supernatant after incubation with the indicated peptide and centrifugation. One hundred percent hemolysis corresponds to the absorbance value of human erythrocytes treated with 0.1% Triton X-100.