Fig. 2.

FGF21 protects mice suppresses inflammation to against septic liver injury. (A) Short-term survival CLP model (n = 8). (B, C) Representative image and quantification of hepatic TUNEL staining of mice in indicated groups (n = 5). Scale bars: 50 μm. (D-F) Liver tissue samples from indicated groups were subjected to H&E staining, and infiltrated cells number around large vessel and small vessel was counted (n = 5). Scale bars: 50 μm. The region enclosed by the dashed lines represents the area infiltrated by inflammatory cells. (G, H) Representative image and quantification of hepatic MPO staining of mice in indicated groups (n = 5). Scale bars: 50 μm. (I) The Serum levels of ALT and AST from mice in indicated groups (n = 5). (J) Elisa analysis for IL-1β, IL-6, and TNF-α in serum of mice in indicated groups (n = 5). (K) The Serum level of FGF21 from mice in indicated groups (n = 5). (L) The expression of FGF21 in liver lysates from wildtype mice and FGF21 KO mice, and normalized to β-actin (n = 4). (M, N) Representative image and quantification of hepatic TUNEL staining of mice in indicated groups (n = 5). Scale bars: 50 μm. (O-Q) Liver tissue samples from indicated groups were subjected to H&E staining, and infiltrated cells number around large vessel and small vessel was counted (n = 5). Scale bars: 50 μm. The region enclosed by the dashed lines represents the area infiltrated by inflammatory cells. (R, S) Representative image and quantification of hepatic MPO staining of mice in indicated groups (n = 5). Scale bars: 50 μm. (T) The Serum levels of ALT and AST from mice in indicated groups (n = 5). (U) Elisa analysis for IL-1β, IL-6, and TNF-α in serum of mice in indicated groups (n = 5). Data are presented as mean ± SEM of each group. Statistical difference in mortality were analyzed by log-rank test. And one-way ANOVA test is using to assess the statistical differences in multiple groups. ns = no significant.