Table 2.
Amounts of adenosine and guanosine phosphates in crude extracts of wild-type E. coli and R. cellulolyticum
| Bacterial species | ADP (nmol) | ATP (nmol) | GDP (nmol) | GTP (nmol) | ATP/GTP |
|---|---|---|---|---|---|
| E. coli MG1655a | 7.07 ± 1.92 | 11.17 ± 2.13 | 6.11 ± 1.55 | 4.6 ± 0.89 | 2.43 ± 0.19 |
| R. cellulolyticumb | 3.95 ± 0.68 | 1.24 ± 0.57 | 1.23 ± 0.45 | 1.04 ± 0.35 | 1.22 ± 0.44 |
aMeans of three biological replicates (10 mL of suspension containing 2.0 ± 0.1 × 109 cells), and two technical replicates for each biological sample. E. coli was grown aerobically in M9 medium supplemented with 2 g/L of glucose.
bMeans of three biological replicates (10 mL of suspension containing 2.0 ± 0.3 × 109 cells) and three technical replicates for each biological sample. R. cellulolyticum was grown anaerobically in basal medium supplemented with 2 g/L of cellobiose.