Figure 6. MBNL1 and/or MBNL2 overexpression results in partial rescue for widespread DGE in ventricles of CUG960 +dox mice.

(A) Gene signature scores for ventricular samples. The center line represents the median value, while the upper and lower dashed lines indicate the first and third quartiles. (B) Differentially expressed genes displaying at least a 1.5-fold change (adjusted P = 0.05) as compared with expression in CUG960 +dox controls. (C) Percentages of overlapping DGE in each MBNL-overexpressing cohort using the comparison between CUG960 +/-dox and +dox cohorts as a reference. (D) GO functional terms for genes showing DGE changes in the ventricles of mice in all cohorts. Dashed line: –log₁₀(FDR) = 1.5. (E–G) RT-qPCR–based validation of candidate genes showing gene expression changes in ventricles of mice in each cohort. Rpl4 was used as an internal control for normalization. n = 3 animals per cohort. Black lines indicate the significant differences in corresponding groups compared with +dox or tdTomato controls; brown lines indicate the significant differences between the corresponding groups and +/–dox and –dox controls. Data represent the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, by ordinary 1-way ANOVA followed by Tukey’s multiple-comparison test. Reg., regulation.