TABLE 2.
Primers used in this study
| Primer | Sequence (5′-3′)a |
|---|---|
| WCFS-lamA-NdeI | cagtgattcatatgTTGACCGGATGTTATCCAG |
| WCFS-lamA-EcoRI | catgaattcATTGGACAAGCACTTGCGACG |
| WCFS-lamA-KpnI | acgggtaccTTAGTAATGATTTGCCG |
| WCFS-lamA-BamHI | aatggatccAGATCATTTGGCGAGTTTAG |
| pBAC-SacB-EcoRI | cgtgaattcTACACTGACTATTCCG |
| pBAC-SacB-KpnI | tcaggtaccGTGTTTGAAGTGATCAGC |
| Lam-NdeI-junction-1 | GTTAGGCCAAGTTACTTTGC |
| Lam-BamHI-junction-4 | CTACGCTTATTGGTACGAGC |
| SacB-control-2 | CATCGATAAACTGCTGAACG |
| SacB-control-3 | CGATGCTGAGTTAGCGAACG |
| RR-lamA-F | GCAGGCGACTTCCTTGAAAC |
| RR-lamA-R | GAAGCGAGTATCTCAGAATG |
| LamBD-NICE-KpnI | CTAGGTACCTTATTTACGTAGTTCGTC |
| LamBD-NICE-NcoI | GGAGTGGGCCATGGAAAAGCCAGAAC |
| pNZ8048-F | GTTAGATACAATGATTTCGTTCG (left of MCS) |
| pNZ8048-R | CAATTGAACGTTTCAAGCCTTGG (right of MCS) |
| Lp1197-2Fb | GACAAGCACGACCAACTCACA |
| Lp1197-2Rb | TAAGCCGACAACTAGCCCAATCAA |
| Lp1204-1Fb | ACGTCATCCATTTCGCTTTTT |
| Lp1204-1Rb | GTCTCATTCCACGCATCTCTGT |
| Lp3581-2Fb | GATTGGCCTATTTGTCATT |
| Lp3581-2Rb | GAGCTTCGATTTCATTCA |
a
Nucleotides homologous to nucleotides in the genome sequence are shown in capital letters, nucleotides in the restriction enzyme recognition sequence are shown in bold type, and nonhomologous nucleotides are shown in lowercase letters. Mismatched nucleotides are in italics; the start codon of LamBD is underlined. MCS, multiple cloning site.
b
Primers used for real-time PCR.