SDS-PAGE and immunoblot analysis of LPS from H. pylori HP0479 isogenic mutants. (A) Lane 1, H. pylori 26695; lane 2, H. pylori 26695::HP479kan; lane 3, H. pylori O:3; lane 4, H. pylori O:3::HP0479kan; lane 5, H. pylori strain SS1; lane 6, H. pylori SS1::HP0479kan; lane 7, H. pylori strain PJ1; lane 8, H. pylori PJ1::HP0479kan. (B) Immunoblot using anti-LeX monoclonal antibody at a 1:250 dilution for lanes 1 to 6 from panel A. (C) Immunoblot using anti-LeY monoclonal antibody at a 1:500 dilution for lanes 1 to 6 from panel A.