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CE-IS-CID-MS (m/z 200 to1400) analysis of delipidated H. pylori LPS, using an orifice voltage of 200 V. (A) Extracted mass spectrum for strain 26695; (B) extracted mass spectrum for 26695::HP0479kan mutant strain; (C) extracted mass spectrum for the complemented 26695::HP0479-HP0480 strain; (D) extracted spectrum in the precursor ion scan for m/z 204. The collision energy was ramped from 40 to 60 V for the scan range of m/z 400 to 1400, with nitrogen as the collision gas.
