Degradation of artificial amorphous PHB granules by PhaZc. The 3HB released during PHB degradation was measured enzymatically. Symbols: ×, no PhaZc; ▴, 0.46 μg of PhaZc; ⧫, 1.2 μg of PhaZc; •, 4.6 μg of PhaZc; ○, 4.6 μg of boiled PhaZc (100°C, 5 min). The error bars indicate standard deviations of the means based on three independent measurements.