TABLE 2.
Effect of algQ, pfrA, and rsd on PpvdA::lacZ activity in E. coli MC4100 expressing the pvdS gene
| Plasmids | LacZ activitya
|
|
|---|---|---|
| M9 medium + 2,2′-dipyridylb | M9 medium + FeCl3b | |
| pMP220::PpvdA + pBR322 | 35 | 31 |
| pMP220::PpvdA + pBRXB | 256 | 27 |
| pMP220::PpvdA + pBRXB + pACYC184 | 214 | 29 |
| pMP220::PpvdA + pBR322 + pACYCalgQ | 32 | 33 |
| pMP220::PpvdA + pBR322 + pACYCpfrA | 35 | 30 |
| pMP220::PpvdA + pBR322 + pACYCrsd | 33 | 31 |
| pMP220::PpvdA + pBRXB + pACYCalgQ | 450 | 40 |
| pMP220::PpvdA + pBRXB + pACYCpfrA | 621 | 51 |
| pMP220::PpvdA + pBRXB + pACYCrsd | 419 | 48 |
a
β-Galactosidase (LacZ) activity is expressed in Miller units (36). The values are means of three independent determinations. The standard deviation is <13% of each value.
b
The M9 minimal medium was supplemented with 150 μM 2,2′-dipyridyl and with 100 μM FeCl3 to reduce and increase iron availability, respectively.