TABLE 3.
Effect of the algQ mutation on the activities of different pvd::lacZ transcriptional fusions in P. aeruginosa PAO1 cells carrying rpoD, pvdS, algQ, ndk, and rsd genes in trans
| Strain | LacZ activitya
|
|||||||
|---|---|---|---|---|---|---|---|---|
| PpvdA::lacZ
|
PpvdD::lacZ
|
PpvdE::lacZ
|
PpvdS::lacZ
|
|||||
| −Fe(III) | +Fe(III) | −Fe(III) | +Fe(III) | −Fe(III) | +Fe(III) | −Fe(III) | +Fe(III) | |
| PAO1(pUCP19) | 7,973 | 45 | 6,402 | 52 | 5,504 | 52 | 4,180 | 58 |
| PAO1(pUCPrpoD) | 5,956 (75 ± 5)b | 28 | 3,559 (56 ± 4) | 31 | 3,242 (59 ± 4) | 29 | 4,431 (106 ± 4) | 51 |
| PAO1(pUCPpvdS) | 8,771 (110 ± 8) | 4,868 | 8,963 (140 ± 6) | 5,333 | 6,808 (123 ± 8) | 4,289 | 4,013 (96 ± 5) | 52 |
| PAO1ΔalgQ(pUCP19) | 5,980 (75 ± 8) | 25 | 4,049 (63 ± 4) | 29 | 3,821 (69 ± 8) | 26 | 4,270 (102 ± 4) | 62 |
| PAO1ΔalgQ(pUCPrpoD) | 3,572 (45 ± 6) | 33 | 2,681 (42 ± 3) | 27 | 2,708 (49 ± 5) | 32 | 4,598 (110 ± 7) | 47 |
| PAO1ΔalgQ(pUCPpvdS) | 8,124 (101 ± 4) | 4,471 | 8,771 (137 ± 8) | 4,959 | 6,671 (121 ± 7) | 4,032 | 3,929 (94 ± 6) | 54 |
| PAO1ΔalgQ(pUCPalgQ) | 7,554 (95 ± 5) | 32 | 5,013 (81 ± 14) | 28 | 4,933 (93 ± 3) | 35 | 4,318 (103 ± 8) | 43 |
| PAO1ΔalgQ(pUCPndk) | 6,177 (77 ± 7) | 31 | 4,081 (64 ± 4) | 30 | 3,928 (71 ± 7) | 33 | 4,265 (102 ± 6) | 55 |
| PAO1(pJB785TT) | 7,863 | 47 | 6,513 | 48 | 5,623 | 36 | 4,092 | 52 |
| PAO1ΔalgQ(pJB785TT) | 5,740 (73 ± 7) | 35 | 4,364 (67 ± 4) | 46 | 3,655 (65 ± 3) | 43 | 4,174 (102 ± 8) | 61 |
| PAO1ΔalgQ(pJBalgQ) | 7,627 (97 ± 5) | 36 | 6,448 (99 ± 3) | 43 | 5,848 (104 ± 6) | 52 | 3,969 (97 ± 5) | 54 |
| PAO1ΔalgQ(pJBrsd) | 6,684 (85 ± 6) | 48 | 5,275 (81 ± 4) | 37 | 4,554 (81 ± 5) | 49 | 3,928 (96 ± 4) | 53 |
a
β-Galactosidase activities were determined in lysates of P. aeruginosa PAO1 cultures grown for 6 h in DCAA [−Fe(III)] and in DCAA supplemented with 100 μM FeCl3 [+Fe(III)]. The values, expressed in Miller units (36), are the means of six independent determinations. The standard deviations are <15% of the means.
b
The values in parentheses (means ± standard deviations) are percentages based on the value obtained for P. aeruginosa PAO1 carrying the control vector (either pUCP19 or pJB785TT), which was considered 100%.