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Analysis of polypeptides by SDS-PAGE. Polypeptides corresponding to full-length HldE (pFM1), HldE2 (ADP-heptose transferase domain; pFM2), HldE1 (heptokinase domain; pFM3), the various mutant forms of HldE, and the GST protein fusion partner (pGEX-2T) are indicated by the arrows. All of the recombinant proteins were detected as GST-fused polypeptides. M, molecular weight markers in thousands.
