FIG. 5.

Citrate increases interaction of RNAP with Pcit in the presence of citrate. (A) Schematic representation of the 260-bp template (amplicon D) used to identify RNAP-DNA-CitI ternary complexes. To simplify the analysis, assays were performed with a DNA fragment including the Pcit promoter (arrow) and O2 (empty rectangle). (B) Electrophoretic mobility shift assays. The experiments were performed with 8 nM of RNAP in the presence (left panel) or absence (right panel) of citrate. In the lanes indicated, CitI was included (+) in the binding reactions. The positions of the different retarded complexes are shown between the panels.