TABLE 1.
Primers used in this study
| Primera | Sequenceb | Coordinate setc |
|---|---|---|
| F(−1285m) | 5′-GTGCAGAATTCTCGTCATCGACGGTGGATAC-3′ | 457-485 |
| F(−253) | 5′-TCTTCGGCAATTTTAGC-3′ | 1489-1505 |
| F(−199m) | 5′-GTGAGAATTCTAATATCATCATACACC-3′ | 1543-1569 |
| F(−199) | 5′-GTGAATGTTGTAATATCATCATACACC-3′ | 1543-1569 |
| F(−169) | 5′-TTAAAAATTTGAACTAGTATGTAAA-3′ | 1573-1597 |
| F(−101) | 5′-ATCAATTGAAAAAGCGTAAGTGC-3′ | 1619-1641 |
| F(−78m) | 5′-GCGAGAATTCAACTGGAAATTCGCAG-3′ | 1664-1689 |
| R(−207m) | 5′-GGATATGCACATATGGATGAAGAACAATTAGC-3′ | 1535-1504 |
| R(−135) | 5′-ATATTTATTTTTTACATACTAGTTC-3′ | 1607-1583 |
| R(−46) | 5′-AAAAAAACTGCGAATTTCCAGTTTAAATCTCG-3′ | 1696-1665 |
| R90 | 5′-TGGGATTTGTGCACCTT-3′ | 1832-1816 |
| R91m | 5′-GTGGGATCCGTGCACCTTCAAAA-3′ | 1833-1811 |
a
The numbers in the primer names indicate the 5′ positions of the primers relative to the transcription start site (+1) of the cit operon (Fig. 2). F, forward primer; R, reverse primer (denotes complementarity to indicated coordinates); m, mutagenic primer.
b
Restriction sites are underlined.
c
Coordinates in pCITJ1 cit region (EMBL nucleotide databank accession number AJ132782).