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Effects of pH on kinetic constants for succinate transport. Km and Vmax values for SdcS are as indicated. Assays were performed as described in Materials and Methods except that assay and wash buffers used morpholineethanesulfonic acid (MES)-MOPS-Tris rather than MOPS-Tris. The concentrations of succinate used to estimate kinetic constants were 1 μM to 200 μM. Kinetic constants are reported as means ± standard errors for three independent experiments.
