TABLE 3.
Primers used in real-time RT-PCR
| Primer | Sequence (5′-3′) |
|---|---|
| SA0123for | ATATTACGGCGAACGGACGAC |
| SA0123rev | TGGCTTGTTATGCTCAAATGAATCG |
| SA0250for | GCATGGTTCTATCGTCGGTACATTG |
| SA0250rev | ACTTACTTTGCGTTTCGGCTTCAC |
| SA0252lrgAfor | TGAAACAACAAAAAGACGCATCAAAACCAG |
| SA0252lrgArev | ACTTCGCCTAACTTAACAGCACCAG |
| SA0270ssaAfor | GGCATCCAAGTCAATTAAACCAAGATAATG |
| SA0270ssaArev | CAGTACGGTAGCTGTTTGTGTTGTAAC |
| SA0319for | GCACCATCTGATATCGAAGTTGAAC |
| SA0319rev | TAGGCGTTCGGCATTTTCAGC |
| SA1248for | TGACAAAGTTGCTGGGCTTGATTAC |
| SA1248rev | TGTGGCTGACGACGTAAAATTGC |
| SA1583for | TCAGCGAGATTGAAAGCGAATAC |
| SA1583rev | CTGTCCATTTCTTTAAGCGTCATAG |
| SA1637for | TGGGGCGGTAAGTATAATGTTTCG |
| SA1637rev | GATCCATTCAATCCACCTGATAAGC |
| SA1882for | GAAAGACAAGCTGGTGCAACAAC |
| SA1882rev | AACGGCGAGAGAAAGTTCATTTAAC |
| SA1844agrAfor | GTGAAATTCGTAAGCATGACCCAGTTG |
| SA1844agrArev | TGTAAGCGTGTATGTGCAGTTTCTAAAC |
| SA1993for | GAAGGAAACAATTGCATTGCTGAAG |
| SA1993rev | ATATCATCACCTTGCGCTTCTTTAG |
| SA1245odhA1665for | GCAATGAACCACCCGTAGAATAGC |
| SA1245odhA1833rev | ACGAGAGCAGCACAAGATGATACAC |
| 16S rRNAfor | CTGTGCACATCTTGACGGTA |
| 16S rRNArev | TCAGCGTCAGTTACAGACCA |