TABLE 4.
Real-time RT-PCR analysis of expression of genes regulated by ArlR
| N315 ORF | N315 gene | N315 description | Change in expression level (n-fold)a
|
|
|---|---|---|---|---|
| RT-PCR | Microarray | |||
| SA0123 | Hypothetical protein | 42.8 | 22.1 | |
| SA0319 | Hypothetical protein | 5.1 | 4.7 | |
| SA1637 | lukD | Leukotoxin LukD | 2 | 1.9 |
| SA1882 | kdpD | Two-component sensor kinase | 3.1 | 2.9 |
| SA1993 | lacF | PTS system, lactose-specific IIA component | 17 | 6.5 |
| SA1844 | agrA | Two-component response regulator | −5.7 | −2.8 |
| SA0250 | lytS | Two-component sensor kinase | −2.8 | −3.1 |
| SA0252 | lrgA | Holin-like protein LrgA | −64.8 | −16.5 |
| SA0270 | ssaA | Secretory antigen precursor SsaA | −1.7 | −2 |
| SA1583 | rot | Repressor of toxin | −4 | −1.9 |
a
Normalized values in the arlR mutant over values in the wild-type strain. Negative numbers denote up-regulation in the wild-type strain.