TABLE 5.
Real-time RT-PCR analysis of expression of genes regulated by ArlR in different growth phases
| N315 ORF | N315 gene | N315 description | Change in expression level (n-fold)a in RT-PCR by phase
|
||
|---|---|---|---|---|---|
| Early log | Mid-log | Stationary | |||
| SA0250 | lytS | Two-component sensor kinase | −4.4 | −7 | −6.7 |
| SA1844 | agrA | Two-component response regulator | −2.7 | −2.3 | −3.9 |
| SA1882 | kdpD | Two-component sensor kinase | −2.6 | 13.8 | 1.2 |
| SA1993 | lacF | PTS system, lactose specific IIA component | 6.3 | 4 | 13 |
| SA1245b | odhA | 2-Oxoglutarate dehydrogenase E1 | NC | NC | NC |
a
Normalized values in the arlR mutant over values in the wild-type strain. Negative numbers denote up-regulation in the wild-type strain.
b
Downstream gene of arlRS. NC, no detectable change.