TABLE 1.
Activity of AldA in strains of M. loti grown on various carbon and nitrogen sources
| Strain | AldA activity (μmol min−1 mg protein−1)a on:
|
||||
|---|---|---|---|---|---|
| Succinate-NH4+ | Alanine | Glucose-NH4+ | Glucose-alanine | Bacteroids | |
| MAFF 303099 | 2.8201 ± 0.92 | 3.0977 ± 0.63 | 0.3810 ± 0.1 | 1.6210 ± 0.43 | 0.382 |
| RU1731 (ΔaldAc) | 0.6895 ± 0.006 | 2.7066 ± 0.46 | 0.04862 ± 0.01 | 0.8879 ± 0.25 | — |
| RU1732 (ΔaldAp) | 1.3152 ± 0.28 | 3.5496 ± 0.82 | 0.3536 ± 0.14 | 0.7871 ± 0.17 | — |
| RU1810 (ΔaldAc ΔaldAp) | ND | ND | ND | ND | ND |
a
Values shown are means from at least three independent replicates ± SEMs. Cultures were grown on acid minimal salts (13) with the carbon and nitrogen sources indicated in the table at concentrations of 10 mM. AldA activity on bacteroids is the average for two independent L. corniculatus nodule harvests and was determined on cultures harvested and assayed as previously described (1). ND, no detectable activity; —, not determined.